[M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 20000 sequences (1400000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 10000, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 500, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (328, 673) [M::mem_pestat] mean and std.dev: (498.32, 49.61) [M::mem_pestat] low and high boundaries for proper pairs: (259, 742) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 20000 reads in 0.220 CPU sec, 0.218 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem refs/AF086833.fa r1.fq r2.fq [main] Real time: 0.252 sec; CPU: 0.228 sec