[bwa_index] Pack FASTA... 0.00 sec [bwa_index] Construct BWT for the packed sequence... [bwa_index] 0.01 seconds elapse. [bwa_index] Update BWT... 0.00 sec [bwa_index] Pack forward-only FASTA... 0.00 sec [bwa_index] Construct SA from BWT and Occ... 0.01 sec [main] Version: 0.7.17-r1188 [main] CMD: bwa index refs/AF086833.fa [main] Real time: 0.329 sec; CPU: 0.020 sec [wgsim] seed = 1554053300 [wgsim_core] calculating the total length of the reference sequence... [wgsim_core] 1 sequences, total length: 18959 [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 20000 sequences (1400000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 9939, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (465, 498, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (333, 663) [M::mem_pestat] mean and std.dev: (497.79, 49.93) [M::mem_pestat] low and high boundaries for proper pairs: (267, 729) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 20000 reads in 0.340 CPU sec, 0.339 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem refs/AF086833.fa r1.fq r2.fq [main] Real time: 0.373 sec; CPU: 0.348 sec