FastQCFastQC Report
Thu 25 Apr 2019
SRR1972918_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1972918_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000
Sequences flagged as poor quality0
Sequence length101
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT110.5499999999999999No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT50.25RNA PCR Primer, Index 26 (100% over 22bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG50.25TruSeq Adapter, Index 1 (96% over 28bp)
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA30.15RNA PCR Primer, Index 27 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)