SRR1972975.trimmed_1U.fq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1972975.trimmed_1U.fq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4705
Sequences flagged as poor quality	0
Sequence length	2-101
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	753	16.00425079702444	RNA PCR Primer, Index 31 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	453	9.628055260361318	RNA PCR Primer, Index 31 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	442	9.394261424017003	RNA PCR Primer, Index 31 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	288	6.121147715196599	RNA PCR Primer, Index 31 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	265	5.63230605738576	RNA PCR Primer, Index 31 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	210	4.463336875664187	RNA PCR Primer, Index 31 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	180	3.825717321997875	RNA PCR Primer, Index 31 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	115	2.4442082890541976	RNA PCR Primer, Index 31 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	115	2.4442082890541976	RNA PCR Primer, Index 31 (96% over 29bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	105	2.2316684378320937	RNA PCR Primer, Index 31 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	93	1.9766206163655684	RNA PCR Primer, Index 31 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	79	1.6790648246546227	RNA PCR Primer, Index 31 (96% over 29bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	71	1.5090329436769394	RNA PCR Primer, Index 31 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	69	1.4665249734325185	RNA PCR Primer, Index 31 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	68	1.445270988310308	RNA PCR Primer, Index 31 (96% over 29bp)
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	62	1.3177470775770457	RNA PCR Primer, Index 38 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAA	57	1.2114771519659937	RNA PCR Primer, Index 31 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	55	1.1689691817215728	RNA PCR Primer, Index 38 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	50	1.0626992561105209	RNA PCR Primer, Index 31 (96% over 29bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCTACGC	48	1.0201912858661	No Hit
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	36	0.7651434643995749	RNA PCR Primer, Index 38 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	35	0.7438894792773645	RNA PCR Primer, Index 31 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAA	33	0.7013815090329437	RNA PCR Primer, Index 31 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	32	0.6801275239107333	RNA PCR Primer, Index 31 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	30	0.6376195536663124	RNA PCR Primer, Index 38 (96% over 28bp)
CTTTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	29	0.6163655685441021	RNA PCR Primer, Index 31 (96% over 29bp)
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	27	0.5738575982996812	RNA PCR Primer, Index 38 (96% over 28bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	24	0.51009564293305	RNA PCR Primer, Index 31 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAA	22	0.46758767268862916	RNA PCR Primer, Index 31 (96% over 29bp)
CTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	22	0.46758767268862916	RNA PCR Primer, Index 38 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAA	20	0.4250797024442083	RNA PCR Primer, Index 31 (96% over 29bp)
ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	20	0.4250797024442083	RNA PCR Primer, Index 38 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAA	19	0.4038257173219979	RNA PCR Primer, Index 31 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAA	18	0.38257173219978746	RNA PCR Primer, Index 31 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	15	0.3188097768331562	RNA PCR Primer, Index 31 (96% over 29bp)
ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	14	0.2975557917109458	RNA PCR Primer, Index 38 (96% over 28bp)
CTTTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	13	0.27630180658873543	RNA PCR Primer, Index 31 (96% over 29bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	13	0.27630180658873543	RNA PCR Primer, Index 31 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAA	12	0.255047821466525	RNA PCR Primer, Index 31 (96% over 29bp)
CTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	12	0.255047821466525	RNA PCR Primer, Index 38 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	11	0.23379383634431458	RNA PCR Primer, Index 38 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAA	11	0.23379383634431458	RNA PCR Primer, Index 31 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAA	11	0.23379383634431458	RNA PCR Primer, Index 31 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAA	11	0.23379383634431458	RNA PCR Primer, Index 31 (96% over 29bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAA	10	0.21253985122210414	RNA PCR Primer, Index 31 (96% over 29bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACGCTACGC	9	0.19128586609989373	No Hit
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	9	0.19128586609989373	RNA PCR Primer, Index 31 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTT	8	0.17003188097768332	RNA PCR Primer, Index 31 (96% over 28bp)
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	8	0.17003188097768332	RNA PCR Primer, Index 38 (96% over 28bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	8	0.17003188097768332	RNA PCR Primer, Index 31 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	7	0.1487778958554729	RNA PCR Primer, Index 38 (96% over 28bp)
ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	7	0.1487778958554729	RNA PCR Primer, Index 38 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG	7	0.1487778958554729	RNA PCR Primer, Index 31 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCTACGCTAT	6	0.1275239107332625	No Hit
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	6	0.1275239107332625	RNA PCR Primer, Index 31 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAA	6	0.1275239107332625	RNA PCR Primer, Index 31 (96% over 29bp)
CTTTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	6	0.1275239107332625	RNA PCR Primer, Index 31 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAA	5	0.10626992561105207	RNA PCR Primer, Index 31 (96% over 29bp)
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	5	0.10626992561105207	RNA PCR Primer, Index 38 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	5	0.10626992561105207	RNA PCR Primer, Index 31 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAA	5	0.10626992561105207	RNA PCR Primer, Index 31 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAA	5	0.10626992561105207	RNA PCR Primer, Index 31 (96% over 29bp)
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAA	5	0.10626992561105207	RNA PCR Primer, Index 38 (96% over 28bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content