FastQCFastQC Report
Wed 26 Sep 2018
SRR1972975_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1972975_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000
Sequences flagged as poor quality0
Sequence length101
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT184918.490000000000002No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT119011.899999999999999RNA PCR Primer, Index 31 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT7397.39RNA PCR Primer, Index 31 (95% over 24bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT2452.45RNA PCR Primer, Index 31 (96% over 27bp)
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG1651.6500000000000001RNA PCR Primer, Index 38 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC1291.29No Hit
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT870.8699999999999999RNA PCR Primer, Index 31 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC610.61No Hit
ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC500.5RNA PCR Primer, Index 38 (96% over 25bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCTACGC490.49No Hit
CTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC490.49No Hit
TATCACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT170.16999999999999998RNA PCR Primer, Index 31 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)