FastQCFastQC Report
Wed 26 Sep 2018
SRR1972973_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1972973_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT3943.94No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT1941.94TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT1251.25RNA PCR Primer, Index 11 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT150.15No Hit
CTTTATAATTAAGCTTTAACGATAGGTCTGGGCTCATATTGTTATTGATA140.13999999999999999No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG130.13TruSeq Adapter, Index 11 (95% over 24bp)
GTATCAAACGGACCCAAAAACATCAGTGGTCAGAGTCCGGCGCGAACTTC120.12No Hit
CTTTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC120.12No Hit
CCCATGTATGATTGAGCAATTCAAAGTGGTTTGGCTGAAACCCTACGAAC120.12No Hit
GTATCTAGTGGGAGAAACATTAAGAGAACACTTGCTGCCATGCCGGAAGA120.12No Hit
ATCCAGGATTATGCTACGCATCCCAAATGCAACAAACGAAGCCAAACCCG110.11No Hit
ATAATAAACTCCGCACTCTTTATAATTAAGCTTTAACGATAGGTCTGGGC110.11No Hit
GTACCAAACGTGTACCGGAACAATTTTTAGAGCAAGAAAACTTTTCTATT110.11No Hit
ATATTTGGCCTCTCTCCCTGCGTGATAATCAAAAAATTCACAATACAGCA110.11No Hit
GCTGAAAAGAAGCCCACCTACAACGAACATTACTTTGAGCACCCTCACAA110.11No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)