FastQCFastQC Report
Wed 30 Sep 2020
SRR519926_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR519926_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000
Sequences flagged as poor quality0
Sequence length251
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAAGGGTTGGTTGTGGCATCCTGCGGTTGATACAGCTTCATTGCCG20.2No Hit
GACCTGGGTACTACCAACTCTTGTGTAGCGATTATGGATGGCACCACTCC20.2No Hit
AACGTTTGCTCGATGATCGCCTGCTCATCGGGGATTAAGCGGGTTTCCAG20.2No Hit
AGCTCCATCGCTTCCTGGTAGGACATCGACTTCAACAACCTCGCATCGGG20.2No Hit
TAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCG20.2No Hit
TGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGC20.2No Hit
GGTCGCGTCCACGATCTGCTGCGCGCGATCGTTGCTCTGCTGTTTCGCCC20.2No Hit
GGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCA20.2No Hit
GTTGTCTGGAATTGTTCCACGGGCCAACGCTGGCATTTAAAGATTTCGGC20.2No Hit
GCGCGGCAAAGAGATCGTCGAGTTGTGACAGATTCGCCATAAAAGCGGCA20.2No Hit
CAAGAGTTGGTAGTACCCAGGTCGATACCAATTATTTTACCCATCTAAAC20.2No Hit
GGCTGCATTAAATCGAGCGGGCGGAGTACGCCATACAAGCCGGAAAGCAT20.2No Hit
CATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAG20.2No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)