FastQCFastQC Report
Wed 30 Sep 2020
bad2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamebad2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length4-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGG15.555555555555555No Hit
CACC15.555555555555555No Hit
CATCGGGGATTA15.555555555555555No Hit
TTTTGATTTTCCCCCGCTGGTGACAGGCATTATTCTCGCTGTCTTTACTCTGCTGGCAATCAC15.555555555555555No Hit
ACAGTTTACGTTCGATAGCCCCTTCGCTCTCCGCCAGCTCTTTCAGCAGTGCC15.555555555555555No Hit
TTCAGGTAATTAACATTCATTTTTTGTGGCTTCTATATTCTGGCGTTAGTCGTC15.555555555555555No Hit
ACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGG15.555555555555555No Hit
ATGG15.555555555555555No Hit
CTTCACCCCCCAGGTGGGTATCACCGTTGGTTGCCAGAACTTCGAA15.555555555555555No Hit
CGTAAG15.555555555555555No Hit
AATGTACAGAACAGTTTTTCTTGCAGTGGACTGATTTTGCCTCGTGGATA15.555555555555555No Hit
CTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTG15.555555555555555No Hit
GCTTCGGCATGACCACCATTCTGCTTAACCTGCACAACGTGGGTTATTTC15.555555555555555No Hit
TCCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGC15.555555555555555No Hit
ATATTGCGGGTGATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGAT15.555555555555555No Hit
TTGA15.555555555555555No Hit
AACAATTCCAGACAACCGACATCGCTTTCAACATTGGCGACCGGAGCCGGGAAGGCAAACGCCGC15.555555555555555No Hit
TGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCAT15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)