FastQCFastQC Report
Wed 7 Oct 2020
SRR1759214_cutadapt.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1759214_cutadapt.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000
Sequences flagged as poor quality0
Sequence length2-50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAGTAATCCAGGATAGGCT4234.2299999999999995No Hit
AAGCTGCCAGTTGAAGAACTGT3153.15No Hit
AACATTCAACGCTGTCGGTGAGT2832.83No Hit
TGAGGTAGTAGGTTGTATAGTT2112.11No Hit
AACATTCAACGCTGTCGGTGAG2102.1No Hit
TGAGATGAAGCACTGTAGCT1181.18No Hit
TTCACAGTGGCTAAGTTCTG1081.08No Hit
TGAGGTAGTAGATTGTATAGTT1011.01No Hit
TCTTTGGTTATCTAGCTGTATGA970.97No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT960.96No Hit
TCCCTGAGACCCTTTAACCTGT930.9299999999999999No Hit
TTCACAGTGGCTAAGTTCTGC800.8No Hit
TCGGATCCGTCTGAGCTTGGCT780.7799999999999999No Hit
TGAGATGAAGCACTGTAGCTCT760.76No Hit
CAACGGAATCCCAAAAGCAGCTG750.75No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACC750.75No Hit
AACCCGTAGATCCGAACTTGTG720.72No Hit
TGAGGTAGTAGTTTGTACAGTT700.7000000000000001No Hit
TGTAAACATCCTTGACTGGAAGCT640.64No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG630.63No Hit
TATTGCACTCGTCCCGGCCTCC610.61No Hit
AACCCGTAGATCCGAACTTGT610.61No Hit
TCCCTGAGACCCTAACTTGTGA610.61No Hit
TGAGATGAAGCACTGTAGCTC590.59No Hit
CACCCGTAGAACCGACCTTGCG580.58No Hit
TGTAAACATCCTCGACTGGAAGCT550.5499999999999999No Hit
GCCTGTCTGAGCGTCGCT550.5499999999999999No Hit
CCTGTCTGAGCGTCGCT510.51No Hit
TCCCTGAGACCCTAACTTGT510.51No Hit
TGTCTGAGCGTCGCT480.48No Hit
TCGAGGAGCTCACAGTCTAGT480.48No Hit
AACCGGGCGGAAACACCA470.47000000000000003No Hit
TCCCTGAGACCCTAACTTGTGAA460.45999999999999996No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT430.43No Hit
TGTAAACATCCCCGACTGGAAGCT420.42No Hit
TGAGGTAGTAGGTTGTATGGTT410.41000000000000003No Hit
TCACAGTGAACCGGTCTCTTT390.38999999999999996No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT380.38No Hit
TGAGATGAAGCACTGTAGCTCA340.33999999999999997No Hit
TGAGGTAGTAGGTTGTATAGT340.33999999999999997No Hit
AGATTTCAACTTAACTTGACCGCTCTGACC340.33999999999999997No Hit
GACACAAGGGTTTGT330.33No Hit
TGAGGTAGTAGGTTGTGTGGTT320.32No Hit
AACTCCAGTCACGCCAATATCTCGTATGC320.32Illumina PCR Primer Index 6 (100% over 29bp)
CATTATTACTTTTGGTACGCG320.32No Hit
TGTAAACATCCTCGACTGGA310.31No Hit
AGCTGGTGTTGTGAATCAGGCCG300.3No Hit
AACATTCAACGCTGTCGGTGA300.3No Hit
CGACACAAGGGTTTGT290.29No Hit
AGCAGCATTGTACAGGGCTATGA290.29No Hit
TCTTTGGTTATCTAGCTGTAT290.29No Hit
TGTAAACATCCTACACTCTCAGCT280.27999999999999997No Hit
TAGCAGCACGTAAATATTGGCG270.27No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTC270.27No Hit
TGAGACCTCTGGGTTCTGAGCT270.27No Hit
AACCCGTAGATCCGAACTTGTGA270.27No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT250.25No Hit
CGCCTGTCTGAGCGTCGCT250.25No Hit
TCACAGTGAACCGGTCTCTTTC250.25No Hit
TCTTTGGTTATCTAGCTGTATG250.25No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT240.24No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTC240.24No Hit
TAGCACCATCTGAAATCGGTT230.22999999999999998No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTG220.22No Hit
TCCAGCATCAGTGATTTTGTT220.22No Hit
TCCAGCATCAGTGATTTTGTTG210.21No Hit
TCGTACGACTCTTAGCGG210.21No Hit
CA200.2No Hit
AACATTCAACCTGTCGGTGAGTT200.2No Hit
TAAAGATTAAGAGAACCAACACCTCTTTACAGTGACC200.2No Hit
TCCCTGAGACCCTTTAACCTGTGA200.2No Hit
TATTGCACTTGTCCCGGCCTGT200.2No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT200.2No Hit
TAGCACCATCTGAAATCGGTTA200.2No Hit
TCCCTGAGACCCTTTAACCTG190.19No Hit
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT190.19No Hit
GTCTGAGCGTCGCT180.18No Hit
TGAGGTAGTAAGTTGTATTGTT180.18No Hit
AACATTCAACGCTGTCGGTGAGTT180.18No Hit
CACCCGTAGAACCGACCTTGC180.18No Hit
GTACAGTACTGTGATAACTGA170.16999999999999998No Hit
AACATTCATTGCTGTCGGTGGGT170.16999999999999998No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGC170.16999999999999998No Hit
TTCAAGTAATTCAGGATAGGTT170.16999999999999998No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT170.16999999999999998No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTC170.16999999999999998No Hit
AACATTCAACCTGTCGGTGAGT170.16999999999999998No Hit
TCGAGGAGCTCACAGTCTAGTA160.16No Hit
AACCGGGCGGAAACACC160.16No Hit
CCACGTTCCCGTGG160.16No Hit
TCCCTGAGACCCTTTAACCTGTG160.16No Hit
TCACAGTGAACCGGTCTCTTTT160.16No Hit
ATAGGCGATAGAAATTGAAACCT160.16No Hit
GGAGATTTCAACTTAACTTGACCGCTCTGACC150.15No Hit
AACCCGTAGATCCGATCTTGT150.15No Hit
CTGTCTGAGCGTCGCT150.15No Hit
CCCCCCACTGCTAAATTTGACTGGCTT150.15No Hit
TTCAAGTAATCCAGGATAGGC150.15No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACT150.15No Hit
TACAGTACTGTGATAACTGACT140.13999999999999999No Hit
TGAGAACTGAATTCCATAGGCTGT140.13999999999999999No Hit
TGAGGTAGTAGATTGTATAGT140.13999999999999999No Hit
TGAGGTAGGAGGTTGTATAGTT140.13999999999999999No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCC130.13No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCT130.13No Hit
CAAAGAATTCTCCTTTTGGGCT130.13No Hit
TACGCCTGTCTGAGCGTCGCT130.13No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCT120.12No Hit
GACACAAGGGTTTG120.12No Hit
GAAGGATCATTA120.12No Hit
ACTTTTAAGGATAACAGCTATCCATTGGTC120.12No Hit
TCCCTGAGACCCTAACTTGTGAAA120.12No Hit
TGAGGTAGTAGGTTGTGTGGTTT110.11No Hit
TATTGCACTTGTCCCGGCCTGTAA110.11No Hit
TCCTGTACTGAGCTGCCCCGAG110.11No Hit
TCGGATCCGTCTGAGCTTGGCTAT110.11No Hit
GTCGCT110.11No Hit
TCGACCGGACCTCGACCGGCT110.11No Hit
TTCAAGTAATCCAGGATAGGCTT110.11No Hit
TCACAGTGAACCGGTCTCTT110.11No Hit
CTAGACTGAAGCTCCTTGAGGA110.11No Hit
TAAGGCACGCGGTGAATGCC110.11No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)