FastQCFastQC Report
Wed 7 Oct 2020
SRR519926.trimmed_2U.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR519926.trimmed_2U.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87
Sequences flagged as poor quality0
Sequence length2-148
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAACCCAACGTGTTGTGATCACTCCCGGCGAGCCCGCCGGGATTGG11.1494252873563218No Hit
GACAGAATAGTGTTTATGCACAGCAGTCAAAATATCAAAAGAAGCCAGCGCAGAGGCCATAGAAAATGTG11.1494252873563218No Hit
TTGCCATCGCTAAAATGATGATGGGATAACGTAACGCTGATTTCACTTTG11.1494252873563218No Hit
TTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGA11.1494252873563218No Hit
GGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGC11.1494252873563218No Hit
GGCCGTAGGTTGTGCGAAAGCGGCACA11.1494252873563218No Hit
GTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGG11.1494252873563218No Hit
CGTCCGGGCGCTCTTTTTCAATAATCTTGCGTACAACTTCCCAGTGAATC11.1494252873563218No Hit
CCCTGCTCAGCCAGTGTTGCTGGACCACTTCCTCGATGA11.1494252873563218No Hit
GCGGCAACAAACAGAACAAAAGGAAAATGGCGGCGATTAAACAGATCGCG11.1494252873563218No Hit
AATGACAGGTATGATAATGCAAATTATAGGCGATGTCCCACAATTGACCG11.1494252873563218No Hit
CAAAAAGTTT11.1494252873563218No Hit
TTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGG11.1494252873563218No Hit
GAGCGGGA11.1494252873563218No Hit
TGGACTACTGCAATGGTGACGAAACCTCGCCATCCCAGCGTTCTTACTCC11.1494252873563218No Hit
TTGAAGCGAGGCATCTGCTCAAGAGCTGCAGCAACGGCTTTCATGATGACGA11.1494252873563218No Hit
CATCGGGGATTAAGCGGGTTTCCAGTTCAAACGGCGTGGTTAGCGCC11.1494252873563218No Hit
CCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAG11.1494252873563218No Hit
CGAA11.1494252873563218No Hit
TATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGGGTCGCAAACGCATTTGGTCGCTAT11.1494252873563218No Hit
ACCGCGCTTTTGCCG11.1494252873563218No Hit
GGCAGCTCCTGCGAAACAGGAAGCGGCAGCGCCGGCACCGGCAGCAAAAG11.1494252873563218No Hit
GAAG11.1494252873563218No Hit
TAAATTTAGCCACCGGCAGATCCAGCATTCCCGCCACCATTGGCACCAGC11.1494252873563218No Hit
CCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCCC11.1494252873563218No Hit
CGACAATCGCTGGGCCGGTTTGGGTATTGAAGATCAGGCGGGC11.1494252873563218No Hit
CGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTT11.1494252873563218No Hit
TCAAGGCTTCTTCTGGTCTGAACGAAGA11.1494252873563218No Hit
CGCCCGCTTCACCTTCGCCCGCAAGACGGA11.1494252873563218No Hit
TCACAACGATATGGCGAAAAACATCGTCAAAGCGGTTGAACGTTG11.1494252873563218No Hit
CCACAACTTCAAATGCCGCATTGGCGCAGAAGTATTTGCACATCGCTGCA11.1494252873563218No Hit
TGGCTATTTTGCAGGATCCACGCGCTTTCCTCGCACAACAGGCGGGCGT11.1494252873563218No Hit
TGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACA11.1494252873563218No Hit
TGTACCACCCCAGCAGCAGCCGGTATCCAGCGCGTATATACCTTCCGGCGTACC11.1494252873563218No Hit
AGGCG11.1494252873563218No Hit
ACAATGCGAC11.1494252873563218No Hit
AATGATTTTTCCCCAATACTGCA11.1494252873563218No Hit
GCTTCGGCATGACCACCATTCTGCTTAACCTGCACAACGTGGGTTATTTC11.1494252873563218No Hit
ACTGGAAAGCAATCCACAGTATGTGGCTCGCGAATCAATCACTCAGTGGCAAA11.1494252873563218No Hit
GAAAGAGCCGGATTATCTGGATATCCCAGCATTCCTGCGTAAGCAAGCTGATTAAGAATTGACTGGAATATG11.1494252873563218No Hit
ATCCCCTTGCCCGGTCAAATGACCGGGCTTTCCGCTATCGTCCACGTCAT11.1494252873563218No Hit
TGACTTAATCGACTTCACGAATATCGACACGCAGCTCTTTCGGCACTTCG11.1494252873563218No Hit
TGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCAT11.1494252873563218No Hit
ATATGAATATTGGAAGAGTTATGTG11.1494252873563218No Hit
GTAGCGTTAACCCGCGCCG11.1494252873563218No Hit
GCGAC11.1494252873563218No Hit
TCCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAA11.1494252873563218No Hit
CTGGTTCCTCATGCAACGATGCCTCACCCGGTTAAAGATGTTCGTGTGTT11.1494252873563218No Hit
GCCCGCACCGATTGATGCCGACGGAAACG11.1494252873563218No Hit
CAGCAAACTGGCGGTGGTAACTGGCAGCAAGCGCAGCGTGGCCCAACGCT11.1494252873563218No Hit
TCCTGATAAAAACCATTCAGGCTGGTTTGTTTACGGAAGGGGGCCGACTG11.1494252873563218No Hit
ATAAACTGGCGGAAGGTATCCGGAAGTTTGCTATTGACCAGG11.1494252873563218No Hit
GTGGCTGATTGTTACCGTCTGCGATGGCAAATTGAACTGGCTTTTAAGCG11.1494252873563218No Hit
CTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGG11.1494252873563218No Hit
ACTGGATACGCT11.1494252873563218No Hit
AAATGCGGGAAATGTTCTTTCACCATCTCTGTCAGTTGCAGGTTGGTTTGCGGAT11.1494252873563218No Hit
CTTCTCCATCATCCACGGACCTTTCTCCGCGCTGGG11.1494252873563218No Hit
AATGTACAGAACAGTTTTTCTTGCAGTGGACTGATTTTGCCTCGTGGATA11.1494252873563218No Hit
CATCG11.1494252873563218No Hit
CGGTCTATGCAA11.1494252873563218No Hit
GAAGTTTTTTG11.1494252873563218No Hit
GCGGCTGGGCCAGAAAAGCATCGCCCAGGTTTTGCACCCATTGCGGGTTT11.1494252873563218No Hit
CCGACATACCACATCGCGGCAATAAAGCTGATGGAAAACGCCCGCTGACG11.1494252873563218No Hit
TGTCTGGCAATCCTAACTCGCTACTGATCCACAACGGCATATCAATA11.1494252873563218No Hit
ATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCA11.1494252873563218No Hit
TACAACCCAAACAATCTGAGCG11.1494252873563218No Hit
GAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGA11.1494252873563218No Hit
CCGATGCGCCAA11.1494252873563218No Hit
ACGGTGGCGCGGTACTTCTGTACCTGCCCCCAGA11.1494252873563218No Hit
ATGGACTTTATGAACCTGAACCAGACTGCACATGGCGGTCGCGAGTTCGGCTTCATTGGCGC11.1494252873563218No Hit
TATGTTTTGCGATTTTTTTTGATATTGATTTGGTGAATATTATTGATCAA11.1494252873563218No Hit
TGATGGGCGAAAACCCGCAATGGGTGCAAAACCTGGGCGATGCTTTTCTG11.1494252873563218No Hit
GCGACACGGTGTTAATTGTGCTGGCGCTGCTGCTGTTGCTGCCACCGTTACTGGCGGTG11.1494252873563218No Hit
AGTCTGGTTGGCGATGCCCATCTCGGTGATCCGTGTCCTGCACGACGCCT11.1494252873563218No Hit
GCCCGCGAACTGGCTCAGCAGCTGGTT11.1494252873563218No Hit
AGCGTCGCGTCATCCGGA11.1494252873563218No Hit
GAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGA11.1494252873563218No Hit
TG11.1494252873563218No Hit
GGCCATGAAGATTACCAGATTGTTGGCAATCAGCGTCTGGACTACTCCGA11.1494252873563218No Hit
TAC11.1494252873563218No Hit
CTTGATCGTCCAAAAGCGAATGCTATTGATGCAAAAACCAGCTTTGAAAT11.1494252873563218No Hit
ACATGCCAGAAGTAATACAAGTGGTTGTAATTGATATGAGACATGCTCAT11.1494252873563218No Hit
TGGGCTACACCAGATATTTTCGATCCGGCCTTCCGTGACGCCC11.1494252873563218No Hit
GCACCGTCATGATGGCGTTCCAGCGGATAGCTGAACGAATACGGCGTTCC11.1494252873563218No Hit
AACTTCAGTTGAGATACGGCCCGGAACCAGTTTCAGGATTTCCAGACCAAT11.1494252873563218No Hit
GCTTCTTCAT11.1494252873563218No Hit
CGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTG11.1494252873563218No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)