Applied Bioinformatics Course Recipes

Results generated by running the recipe.

Output Messages
Messages printed to the standard output stream:

Building a new DB, current time: 10/21/2020 12:11:09
New DB name:   /export/www/biostar-central/export/media/jobs/job-1299/db/ebov_prots
New DB title:  db/proteins.fa
Sequence type: Protein
Keep MBits: T
Maximum file size: 1000000000B
Adding sequences from FASTA; added 2240 sequences in 0.0535531 seconds.


Building a new DB, current time: 10/21/2020 12:11:09
New DB name:   /export/www/biostar-central/export/media/jobs/job-1299/db/ebov_genomes
New DB title:  db/genomes.fa
Sequence type: Nucleotide
Keep MBits: T
Maximum file size: 1000000000B
Adding sequences from FASTA; added 249 sequences in 0.0344551 seconds.
Database: db/proteins.fa
	2,240 sequences; 1,367,025 total residues

Date: Oct 21, 2020  12:11 PM	Longest sequence: 2,212 residues

BLASTDB Version: 4

Volumes:
	/export/www/biostar-central/export/media/jobs/job-1299/db/ebov_prots
Other Messages
Messages printed to the standard error stream:
+ mkdir -p db
+ esearch -db protein -query PRJNA257197
+ efetch -format fasta
+ esearch -db nuccore -query PRJNA257197
+ efetch -format fasta
+ makeblastdb -in db/proteins.fa -dbtype prot -out db/ebov_prots -parse_seqids
+ makeblastdb -in db/genomes.fa -dbtype nucl -out db/ebov_genomes -parse_seqids
+ blastdbcmd -db db/ebov_prots -info
+ echo '>mystery'
+ echo ATGGACTCTCGTCCTCAGAAAGTCTGGATGACGCCGAGTCTCACTGAATCTGACATGGAT
+ echo TACCACAAGATCTTGACAGCAGGTCTGTCCGTTCAACAGGGGGTTGTTCGGCAAAGAGTC
+ echo ATCCCAGTGTATCAAGTAAACAATCTTGAG
+ F='6 qacc sacc pident qlen length frames'
+ blastn -db db/ebov_genomes -query foo.fa -outfmt '6 qacc sacc pident qlen length frames'
+ sort -k 3rn
+ blastx -db db/ebov_prots -query foo.fa -outfmt '6 qacc sacc pident qlen length frames'
+ sort -k 3rn
+ tblastx -db db/ebov_genomes -query foo.fa -outfmt '6 qacc sacc pident qlen length frames'
+ sort -k 3rn

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