Qiime2 classification validator

Qiime2 classification validator

The recipe generates simulated sequencing reads from NCBI accession numbers. It then performs class…

1 result • updated 3 months ago by Aswathy

The recipe generates simulated sequencing reads from NCBI accession numbers. It then performs classification with a machine-learning based tool available in Qiime2.

In the third step evaluates and reports the accuracy (the percent of reads were classified correctly) of the classification.

The input to the recipe is a list of NCBI accession numbers:

AP012081
AP011279
DQ288268
AP014537

The output is a table with a taxonomic assignment of each read.

accession   expect  actual  percent taxid   title
MG570454    1000    25      2.5     90988   Pimephales promelas isolate NEFC F17-076 mitochondrion, complete genome
MG570425    1000    40      4.0     28800   Notemigonus crysoleucas isolate NEFC F16-041 mitochondrion, complete genome
MG570424    1000    48      4.8      7971   Catostomus commersonii isolate NEFC F16-032 mitochondrion, complete genome
MG570419    1000    48      4.8     67558   Semotilus atromaculatus voucher NEFC F16-365 mitochondrion, complete genome
MG570417    1000    29      2.9    407093   Rhinichthys obtusus voucher NEFC F16-301 mitochondrion, complete genome
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