Use SAM flags to make scientific discoveries. (work in progress)
This recipe follows a publication titled Genome-wide antisense transcription drives mRNA processing in bacteria published in PNAS, 2011.
The data was published under the Bioproject: PRJNA80817
The recipe is meant to demonstrate the use of alignments and samtools to find evidence of antisense transcription in Staphylococcus aureus.
In addition, the recipe is meant to train you on identifying the properties of sequencing data from the alignments.
The recipe is not quite ready yet, but already contains useful information.